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Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
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Biologia
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JoVE Journal Biologia
Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

DOI:
10.3791/1155-v

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09:12 min

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July 09, 2009

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, , ,
1Department of Zoology,University of British Columbia – UBC

Capítulos

  • 00:00Título
  • 00:57Introduction
  • 01:28Pre-experiment Set Up
  • 03:13Dissection of the Drosophila Eye-Brain Complex
  • 06:22Mounting the Eye Imaginal Disc in a Magnetic Culture Chamber
  • 07:12Visualization of Migrating Glial Cells in the Eye Imaginal Disc
  • 07:41Representative Results: Time-lapse Confocal Images
  • 08:53Conclusion

Summary

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Here we describe a protocol to examine the migration of glial cells into the developing Drosophila eye using live microscopic analysis paired with GFP tagged glial cells.

Tags

Live ImagingGlial Cell MigrationDrosophila Eye Imaginal DiscVertebrate OrganismsInvertebrate OrganismsFinal Target RegionsEnsheath And Support NeuronsLive MigrationDeveloping Pupal WingMicroscopic AnalysisMotile CellsCellular BehaviorRate Of GrowthChanges In DirectionGenetic ToolsSpecific GenesDevelopment ProcessOptic StalkEye Imaginal DiscGFP ExpressionWild TypeMutant Animals

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