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线虫神经肌肉交界处的解剖和录制
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Biologia
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JoVE Journal Biologia
Dissecting and Recording from The C. Elegans Neuromuscular Junction
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线虫神经肌肉交界处的解剖和录制

DOI:
10.3791/1165-v

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15:19 min

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February 25, 2009

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1Department of Biological Sciences,University of Illinois, Chicago

Capítulos

  • 00:00Título
  • 01:11Introduction
  • 01:36Preparing for the dissection
  • 04:37The dissection
  • 09:15Patch-clamping muscle cells
  • 11:11Modes of Muscle Stimulation
  • 13:10Representative Results
  • 14:41Conclusion

Summary

Tadução automática

Tadução automática

电的应用访问突触的突触活动提供量化的测量，分析突触的突变体。本文介绍了一种夹层的方法，用来揭露神经肌肉接头（NMJ）<em>线虫（线虫）</em并简要讨论了一些可用于本制剂的使用。

Tags

Neuromuscular JunctionNeurotransmissionNeuronsChemical SignalsPost-synaptic TargetsPre-synaptic ProteinsPost-synaptic ProteinsSynaptic ConnectivityElectrical SignalsSecretory VesiclesCalcium SensingVesicle FusionPostsynaptic ReceptorsRecycling MechanismsElectrophysiological RecordingsSynaptic TransmissionMolecular ToolsGenetic ToolsC. Elegans NMJsMotor NeuronsBody Wall MusclesLocomotionUnc Mutants

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