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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
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JoVE Journal Biologia
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

DOI:
10.3791/1168-v

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12:00 min

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April 08, 2009

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, ,
1Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Capítulos

  • 00:00Título
  • 00:29Introduction
  • 01:06Setting Up the Infection
  • 02:33Infecting Cells
  • 04:08Harvesting Cells
  • 05:38RNA extraction of samples in TRIzol
  • 10:11Results: Agilent BioAnalyzer Data
  • 10:43Conclusion

Summary

Tadução automática

Tadução automática

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 1 of 3.

Tags

Vaccinia Virus InfectionTemporal AnalysisVirus Gene ExpressionPoxviridaeOrthopox GenusVariolaSmall PoxMonkeypoxVaccinia (VAC)Virus FamilyVaccinia GenomeEarly GenesIntermediate GenesLate GenesVirally-encoded RNA PolymerasesHost Cellular Environment ModulationTranscript Abundance AnalysisTime Course InfectionsHeLa CellsRNA Isolation And AmplificationMicroarray AnalysisGene Expression Analysis

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