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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
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JoVE Journal Biologia
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3

DOI:
10.3791/1170-v

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07:35 min

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April 13, 2009

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, ,
1Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Capítulos

  • 00:00Título
  • 00:45aRNA Labeling: Amino Allyl Coupling of the Dyes
  • 02:30Labeled aRNA Clean-up
  • 06:02Resultados
  • 06:33Conclusion

Summary

Tadução automática

Tadução automática

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 3 describes the process of fluorescently labeling the amplified RNA from both host and viral samples by amino allyl coupling of dyes. Part 3 of 3.

Tags

Vaccinia Virus InfectionTemporal AnalysisVirus Gene ExpressionPoxviridae FamilyOrthopox GenusVariolaSmall PoxMonkeypoxVaccinia (VAC)Virus GenomeEarly GenesIntermediate GenesLate GenesViral RNA PolymerasesHost Cellular Environment ModulationTranscript Abundance AnalysisHeLa CellsTime Course InfectionsRNA IsolationRNA AmplificationMicroarray AnalysisGene Expression

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