Journal
/
Biologia
/
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

DOI:
10.3791/1266-v

•

07:03 min

•

July 02, 2009

•

, , , ,
1Miltenyi Biotec,GmbH

Capítulos

  • 00:04Título
  • 00:31Introduction
  • 01:01Preparing Enzyme Solutions and Buffers
  • 01:50Dissociating the Lung Tissue
  • 04:10Filtration
  • 05:35Representative Results
  • 06:07Conclusion

Summary

Tadução automática

Tadução automática

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

Tags

StandardizedPreparationSingle-cell SuspensionsGentleMACS DissociatorExperimentsCellular ResearchDissociatingWhole OrgansViable CellsReproducible MannerInstrumentPre-programmed SettingsOptimizedEfficient DissociationMouse LungsPublication

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Generation of Single-Cell Suspensions from Mouse Neural Tissue

Single-cell Suction Recordings from Mouse Cone Photoreceptors

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Generation of Mice Derived from Induced Pluripotent Stem Cells

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Read Article