Journal
/
Biologia
/
标准化的制备小鼠肺组织单细胞悬浮液使用gentleMACS Dissociator
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Please note that all translations are automatically generated. Click here for the English version.

标准化的制备小鼠肺组织单细胞悬浮液使用gentleMACS Dissociator

DOI:
10.3791/1266-v

•

07:03 min

•

July 02, 2009

•

, , , ,
1Miltenyi Biotec,GmbH

Capítulos

  • 00:04Título
  • 00:31Introduction
  • 01:01Preparing Enzyme Solutions and Buffers
  • 01:50Dissociating the Lung Tissue
  • 04:10Filtration
  • 05:35Representative Results
  • 06:07Conclusion

Summary

Tadução automática

Tadução automática

游离特定的组织类型的细胞，组织鼓动的具体参数，以获得一个可行的，可培养细胞的高容量。美天旎gentleMACS dissociator优化这个任务简单，实用的协议。在本出版物中使用本仪器对肺组织进行了解释。

Tags

StandardizedPreparationSingle-cell SuspensionsGentleMACS DissociatorExperimentsCellular ResearchDissociatingWhole OrgansViable CellsReproducible MannerInstrumentPre-programmed SettingsOptimizedEfficient DissociationMouse LungsPublication

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/1266?article_type=v

gentleMACS Dissociator从小鼠脾脏单细胞悬浮液的制备
check_url/pt/1266?article_type=v

从小鼠神经组织产生单细胞悬浮液
check_url/pt/1266?article_type=v

单从小鼠锥感光细胞吸录音
check_url/pt/1266?article_type=v

从细胞中一氧化氮和超氧负离子自由基的电子顺磁共振光谱的检测，使用自旋陷阱
check_url/pt/1266?article_type=v

诱导多能干细胞来源于小鼠的生成
check_url/pt/1266?article_type=v

简单和高效的睾丸细胞悬液的制备技术
check_url/pt/1266?article_type=v

尼罗罗非鱼肠单细胞悬液制备用于单细胞测序
check_url/pt/1266?article_type=v

从快速冷冻肝组织中分离细胞核用于单细胞多组学
check_url/pt/1266?article_type=v

使用组织解离器从小鼠白色脂肪组织中半自动分离基质血管部分
check_url/pt/1266?article_type=v

从小鼠颈动脉制备用于单细胞测序的单细胞悬液

Read Article