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Dendra2 Photoswitching through the Mammary Imaging Window
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Biologia
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JoVE Journal Biologia
Dendra2 Photoswitching through the Mammary Imaging Window

Dendra2 Photoswitching through the Mammary Imaging Window

DOI:
10.3791/1278-v

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09:12 min

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June 05, 2009

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, , , ,
1Department of Anatomy and Structural Biology,Albert Einstein College of Medicine – Yeshiva University, 2Gruss Lipper Biophotonics Center,Albert Einstein College of Medicine – Yeshiva University, 3Hubrecht Institute-KNAW and University Medical Center Utrecht

Capítulos

  • 00:05Título
  • 00:25Introduction
  • 01:03Manual Fabrication of the Mammary Imaging Window Window (MIW)
  • 02:58Insertion of MW
  • 05:28Using the Imaging Box
  • 06:39Photoswitching and Imaging of Dendra2-labeled Cells
  • 08:09Representative Results: Photoswitching
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

Tags

Intravital MicroscopyBreast TumorsMiceRatsSingle-cell ResolutionMetastatic BehaviorMigrationInvasionIntravasationAngiogenesisImmune Cells ResponseMammary CarcinomasMammary Imaging Window (MIW)Imaging ParametersDendra2 PhotoswitchingIn Vivo ImagingProtocolManufacturing MIWTumor Cells ImagingCustom Built Imaging BoxMetastatic Behavior

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