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在整个猴脑进行大规模蛋白质检测的批次免疫染色
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JoVE Journal Biologia
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
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在整个猴脑进行大规模蛋白质检测的批次免疫染色

DOI:
10.3791/1286-v

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08:07 min

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July 27, 2009

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, , ,
1Cognitive Neuroscience Unit,Montreal Neurological Institute, 2Ècole d’Optomètrie,Universitè de Montrèal, 3Department of Psychology,McGill University

Capítulos

  • 00:05Título
  • 00:54Introduction
  • 01:46Tissue Preparation
  • 02:55Histological Processing
  • 05:34Mounting Sections onto Slides
  • 06:58Representative Results
  • 07:25Conclusion

Summary

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Tadução automática

大型整个灵长类动物大脑的靶蛋白的免疫检测是采用新颖的组织包埋，切片与创意仪器在某一特定时间使用多个自由浮动的部分批次的染色相结合的方法。

Tags

Batch ImmunostainingLarge-scale Protein DetectionWhole Monkey BrainImmunohistochemistry (IHC)Target ProteinsExpression PatternBrainRodentsPrimatesSystematic ApproachImmunodetectionAdult Monkey BrainSectioning MethodologyNeuroscience Associates (NSA)Batch-stainingFree-floating SectionsUniform StainingAnalytical ProceduresProtein LevelsNeurons Expressing Protein

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