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Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
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Biologia
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JoVE Journal Biologia
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

DOI:
10.3791/1286-v

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08:07 min

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July 27, 2009

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, , ,
1Cognitive Neuroscience Unit,Montreal Neurological Institute, 2Ècole d’Optomètrie,Universitè de Montrèal, 3Department of Psychology,McGill University

Capítulos

  • 00:05Título
  • 00:54Introduction
  • 01:46Tissue Preparation
  • 02:55Histological Processing
  • 05:34Mounting Sections onto Slides
  • 06:58Representative Results
  • 07:25Conclusion

Summary

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Tadução automática

Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.

Tags

Batch ImmunostainingLarge-scale Protein DetectionWhole Monkey BrainImmunohistochemistry (IHC)Target ProteinsExpression PatternBrainRodentsPrimatesSystematic ApproachImmunodetectionAdult Monkey BrainSectioning MethodologyNeuroscience Associates (NSA)Batch-stainingFree-floating SectionsUniform StainingAnalytical ProceduresProtein LevelsNeurons Expressing Protein

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