É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:37Introduction
- 01:22Preparation
- 03:42Preparation of Hemolymph
- 05:33Dissecting the Ganglia from Aplysia
- 10:05Removing and Culturing Neurons from the Pleural Ganglia
- 12:25Isolating Motor Neurons and Preparing Co-cultures with Sensory Neurons
- 16:53Conclusion
Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in culture.
