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Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
JoVE Journal
Biologia
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JoVE Journal Biologia
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
DOI:

16:44 min

June 02, 2009

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Capítulos

  • 00:05Título
  • 00:55Introduction
  • 01:49An Optical Projection Tomography Microscope
  • 04:51Preparing Tissue for Tomographic imaging
  • 09:30Absorption Reconstructions
  • 11:36Fluorescent Reconstructions
  • 15:44Conclusion

Summary

Tadução automática

We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.

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