Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Claudio Vinegoni; Daniel Razansky; Jose-Luiz Figueiredo; Lyuba Fexon; Misha Pivovarov; Matthias Nahrendorf; Vasilis Ntziachristos; Ralph Weissleder

doi:10.3791/1389

Journal

/

Biologia

/

生于正常化荧光整个心脏成像的光学投影层析成像

JoVE Journal
Biologia

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JoVE Journal Biologia

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

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生于正常化荧光整个心脏成像的光学投影层析成像

DOI:

10.3791/1389-v

•

16:44 min

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June 02, 2009

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Claudio Vinegoni², Daniel Razansky, Jose-Luiz Figueiredo², Lyuba Fexon², Misha Pivovarov², Matthias Nahrendorf², Vasilis Ntziachristos, Ralph Weissleder²

¹Center for Systems Biology,Harvard Medical School, ²Center for Systems Biology,MGH – Massachusetts General Hospital, ³Institute for Biological and Medical Imaging,Technical University of Munich and Helmholtz Center Munich

Capítulos

00:05Título
00:55Introduction
01:49An Optical Projection Tomography Microscope
04:51Preparing Tissue for Tomographic imaging
09:30Absorption Reconstructions
11:36Fluorescent Reconstructions
15:44Conclusion

Summary

Tadução automática

我们建议一个光学投影层析成像出生的规范化方法（BnOPT），占成像样品的吸收性能，以获得准确，定量荧光断层重建。我们利用该算法重建的荧光分子探针分布在小动物内脏。

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