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Capítulos
- 00:00Título
- 00:55Introduction
- 01:42Preparing Lectin-Conjugated POROS Columns
- 05:15Packing the Lectin-POROS Beads into a PEEK Column
- 08:11HPLC of Samples
- 11:49Desalting the Collected Fractions and PNGase F-digestion
- 14:50Ziptip Desalting of PNGaseF Samples
- 16:34Glycopeptide Analysis Representative Results
- 19:45Conclusion
Lektin-konjuge Poros boncuk HPLC için istihdam edildi. Glikopeptid standartları, pozitif ve negatif kontroller olarak görev yaptı. MARS-14 tükenmiş, tripsin-sindirilmiş insan plazma Kromatografi ve flow-through (FT) ve bağlı kesirler ESI-LC-MS/MS analizler için toplanmıştır. FT'ye göre glikopeptid bağlı fraksiyon zenginleştirilmiş.
Tags
Lectin HPLC MethodSelectively-glycosylated PeptidesComplex Biological SamplesGlycansPost-translational ModificationsTumor CellsCancer-associated Glycan VariantsBiomarker DevelopmentLectinsSambucus Nigra (SNA)Aleuria Aurantia (AAL)Affinity MatricesPOROS BeadsPEEK ColumnsHigh Pressure Liquid Chromatography (HPLC)Plasma DepletionMultiple Affinity Removal System (MARS-14)Trypsin-digestionFlow-through FractionBound FractionPNGaseFN-linked GlycansLC-MS/MS AnalysisQStar EliteMascot SoftwarePositive ControlsNegative Controls
