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Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
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JoVE Journal Biologia
Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

DOI:
10.3791/1405-v

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16:19 min

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September 01, 2009

•

,
1Unitat de Biologia Cel·lular (Facultat de Biociències),Universitat Autónoma de Barcelona

Capítulos

  • 00:05Título
  • 00:58Part 1: Processing and Sample Fixation
  • 04:17Part 2: Decondensation
  • 06:55Part 3: Hybridization
  • 09:35Part 4: Washes post-hybridization
  • 12:11Part 5: Visualization
  • 14:21Conclusions

Summary

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This video-article describes, step by step, how to process a semen sample to achieve good-quality fluorescence in situ hybridization on human spermatozoa. Preparations obtained can be used for aneuploidy screening in the context of clinical diagnosis.

Tags

Fluorescence In Situ HybridizationFISH ProtocolHuman SpermAneuploidiesChromosomal AbnormalitiesMeiotic ErrorsGametogenic ProcessNumerical Chromosome AbnormalitiesOffspringClinical DiagnosisGenetic Reproductive AdviceChromosomes XY131821Semen Sample ProcessingSperm Chromatin Decondensation And DenaturationSperm FISH PreparationsMicroscope Visualization

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