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取得高纯度弓形虫卵囊

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

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取得高纯度弓形虫卵囊

DOI:

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11:17 min

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November 03, 2009

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Sarah E. Staggs, Mary Jean See, J P. Dubey, Eric N. Villegas⁴

¹Dynamac, Inc., ²Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, ³Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, ⁴National Exposure Research Laboratory,US Environmental Protection Agency

Capítulos

00:00Título
01:05Introduction
01:35General Safety When Working With T. gondii Oocysts
03:15Protocol Preparation and the Sucrose Float
07:27CsCl Gradient
10:03Resulting Oocysts
10:34Conclusion

Summary

Tadução automática

这项研究描述了修改后的铯的方法，可以轻松地净化适用于分子生物学和组织培养操作感染的猫粪的弓形虫卵囊的发展

Tags

Toxoplasma Gondii Oocysts Cesium Chloride Gradient Protozoan Pathogen Food-borne Disease Outbreaks Feline Species Sexually Replication Infectious Oocyst Contaminated Meat Contaminated Soil Contaminated Water Latent Infection Toxoplasmic Encephalitis Immunocompromised Seroprevalence Waterborne Outbreaks

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