This content is Free Access.
Capítulos
- 00:00Título
- 01:22Introduction
- 02:01Preparing the Microinjection Plate and Needles
- 06:03Microinjection of Fluorescent Alexa Fluor® 594 Dextran
- 07:03Embryo and Transplant Apparatus Setup
- 10:01Gastrula Stage Cell Transplants
- 13:26Visualizing and Imaging Embryos
- 15:31Gastrula Cell Transplantation Results
- 18:08Conclusion
Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.
Tags
Zebrafish GastrulaCell TransplantationBiologia do DesenvolvimentoCell FunctionsProtein MisexpressionForebrain CommissuresNeuron-glial Cell InteractionsAstroglial PopulationsGuidance CuesProjecting AxonsMidline CrossingZebrafish EmbryosEctopic Cell PlacementGene MisexpressionVital Dye TracerFluorescent Protein Expression
