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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
JoVE Journal
Biologia
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JoVE Journal Biologia
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

DOI:
10.3791/1432-v

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14:36 min

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August 26, 2009

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, , ,
1Mechanical Engineering,Johns Hopkins University, 2Biomedical Engineering,Duke University, 3Biomedical Engineering,Johns Hopkins University

Capítulos

  • 00:05Título
  • 00:54Introduction
  • 01:23Biotinylation of DNA and Labeling of the Cy5-Cationic Polymer
  • 05:51Preparing the QD-labeled DNA and Cy5-Polymer
  • 07:15Fabricating the SU-8 Masters Using Standard Photolithography
  • 10:07Replica Molding of PDMS from the Masters and Bonding to the Cover Glass
  • 12:16Monitoring the Formation of DNA Nanocomplexes in the Microfluidic Device
  • 13:30Results of QD-FRET-Indicated DNA Nanocomplex Self-Assembly
  • 14:04Conclusion

Summary

Tadução automática

Tadução automática

We present a novel and powerful integration of nanophotonics (QD-FRET) and microfluidics to investigate the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

Tags

QD-FRETMicrofluidicsDNA Nanocomplex Self-assemblyGenomicsTherapeuticsIntracellular Delivery SystemGene CarrierElectrostatic Self-assemblyCellular UptakeDegradation ProtectionRational DesignPremature DissociationStable BindingSelf-assembly KineticsPhysical PropertiesTransfection EfficienciesBioactivitiesNanophotonics

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