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Capítulos
- 00:05Título
- 00:54Introduction
- 01:23Biotinylation of DNA and Labeling of the Cy5-Cationic Polymer
- 05:51Preparing the QD-labeled DNA and Cy5-Polymer
- 07:15Fabricating the SU-8 Masters Using Standard Photolithography
- 10:07Replica Molding of PDMS from the Masters and Bonding to the Cover Glass
- 12:16Monitoring the Formation of DNA Nanocomplexes in the Microfluidic Device
- 13:30Results of QD-FRET-Indicated DNA Nanocomplex Self-Assembly
- 14:04Conclusion
Vi presenterar en ny och kraftfull integration av nanofotonik (QD-FRET) och mikrofluidik att undersöka bildandet av polyelektrolyt polyplexes, vilket förväntas ge bättre kontroll och syntes av enhetliga och anpassningsbara polyplexes för framtida nukleinsyra-baserade läkemedel.
Tags
QD-FRETMicrofluidicsDNA Nanocomplex Self-assemblyGenomicsTherapeuticsIntracellular Delivery SystemGene CarrierElectrostatic Self-assemblyCellular UptakeDegradation ProtectionRational DesignPremature DissociationStable BindingSelf-assembly KineticsPhysical PropertiesTransfection EfficienciesBioactivitiesNanophotonics
