É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:44Introduction
- 01:02Growing Isotopically Light and Isotopically Heavy Yeast Cells
- 03:06Lysing Cells
- 06:35Isolation and Fractionation of Peptides
- 06:38Alkylation and Reduction
- 10:32Desalting
- 11:54Peptide Fractionation
- 13:11Enrichment of Phosphopeptides
- 14:51Representative Results
- 15:14Conclusion
Tags
Quantitative PhosphoproteomicsFatty Acid StimulationSaccharomyces CerevisiaeGrowth And StimulationOleateIsotopically Heavy And Light CellsCryolysis ProcedureBall Mill GrinderUrea SolubilizationMetabolically Inactive StatePhosphorylation PreservationPreventing ReorientationCell LysisReductionAlkylationTrypsin DigestionDesalting On C18 ColumnsSample Complexity ReductionFractionation Using Hydrophilic Interaction Chromatography (HILIC)Phosphopeptides Elution ProfileImmobilized Metal ChromatographyCharge-based AffinitiesMass Spectrometry Analysis
