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An In vitro FluoroBlok Tumor Invasion Assay

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

An In vitro FluoroBlok Tumor Invasion Assay

An In vitro FluoroBlok Tumor Invasion Assay

DOI:

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07:53 min

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July 20, 2009

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Jeff Partridge, Paula Flaherty

¹BD Biosciences,Discovery Labware

Capítulos

00:05Título
00:59Introduction
01:22Preparing Cells and the BD Tumor Invasion System
03:30Cell Invasion, Labeling Cells, and Measuring Fluorescence
05:59Cell Invasion Assay Results
07:27Conclusion

Summary

Tadução automática

This video demonstrates how to measure cell invasion through cell culture inserts using a fluorescence-based methodology.

Tags

In Vitro FluoroBlok Tumor Invasion Assay Metastatic Cells Basement Membrane Migrate Invasive Property BD BioCoat Tumor Invasion System BD Falcon FluoroBlok 24-Multiwell Insert Plate 8.0 Micron Pore Size PET Membrane BD Matrigel Matrix Reconstituted Basement Membrane Protein Structure Invasion Study Cell Invasion Quantitation Fluorescent Dye Labeling DiIC12(3)Calcein AM Fluorescence Measurement BD FluoroBlok Membrane

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