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病毒融合动力学测量方法在单粒子能级

JoVE Journal
Biologia

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JoVE Journal Biologia

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

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病毒融合动力学测量方法在单粒子能级

DOI:

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14:59 min

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September 07, 2009

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Daniel L. Floyd, Stephen C. Harrison², Antoine M. van Oijen

¹Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School, ²Howard Hughes Medical Institute,Harvard Medical School

Capítulos

00:04Título
00:49Introduction
01:12Preparing Dextran-Functionalized Coverslips
04:29Preparing the Liposomes
06:41Setting Up the Microfluidic Flow Cell
08:28Labeling the Virus Particles
10:16Observing Viral Fusion Kinetics Under the Microscope
13:10Viral Hemifusion and Pore Formation Data: Microscope Footage, Fluorescence Intensity Trajectories, and Lag Times for Viral Hemifusion and Pore Formation
14:30Conclusion

Summary

Tadução automática

我们提出了一个<em在体外，</em>两色荧光分析法的可视化与流体的目标双层单个病毒粒子的融合。通过标记荧光基团的差异染色病毒的细胞膜和其内部的病毒颗粒，我们是能够监测hemifusion和孔隙形成的动力学。

Tags

Viral Fusion Kinetics Single Particle Level Membrane Fusion Fusion Assay Fluorescence Assay Fluorphore Staining Ganglioside-containing Lipid Bilayer CCD Camera PH-dependent Fusion Hemifusion Pore Formation

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