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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
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Biologia
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JoVE Journal Biologia
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

DOI:
10.3791/1485-v

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08:44 min

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October 29, 2009

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, ,
1School of Biological Sciences,Nanyang Technological University, Singapore – NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

Capítulos

  • 00:00Título
  • 00:08Introduction
  • 00:52Gel Preparation
  • 02:37Prerun
  • 04:03Load Samples and Run the Gel
  • 05:43Process the gel

Summary

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Denaturing urea polyacrylamide gel electrophoresis is used to separate single-stranded DNA or RNA up to a limit of 500 nucleotides. Urea in combination with heat denatures samples and unstructured single strands migrate within the gel matrix according to their molecular weight.

Tags

Denaturing Urea Polyacrylamide Gel ElectrophoresisUrea PAGEDNA SeparationRNA SeparationPolyacrylamide Gel MatrixMolecular Weight SeparationAcrylamide ConcentrationLower Molecular Weight FragmentsUnstructured DNA Or RNA MoleculesSingle Stranded DNASingle Stranded RNAOligonucleotidesEnzymatic Cleavage ReactionsGel PreparationGel Running

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