Lentivirus Production

Xiaoyin Wang; Michael McManus

doi:10.3791/1499

Journal

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Biologia

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Lentivirus Production

JoVE Journal
Biologia

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JoVE Journal Biologia

Lentivirus Production

DOI:

10.3791/1499-v

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11:42 min

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October 02, 2009

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Xiaoyin Wang, Michael McManus

¹Diabetes Center,University of California, San Francisco – UCSF

Capítulos

00:00Título
00:46Introduction
01:15Transfection of 293 Cells To Produce Lentivirus
03:24Lentivirus Harvest
04:39Lentivirus Concentration via Ultracentrifugation
07:24Lentivirus Titration Using Flow Cytometry
11:17Conclusion

Summary

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To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.

Lentivirus Production

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Capítulos

Summary

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