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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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Biologia
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JoVE Journal Biologia
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

DOI:
10.3791/1508-v

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11:26 min

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September 08, 2009

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, , ,
1Bioquímica,University of Oxford, 2Física,University of Oxford

Capítulos

  • 00:04Título
  • 01:02Introduction
  • 01:41Preparation of Bacteria
  • 03:49Preparing Cells for Microscopy
  • 07:16Visualizing and Analyzing the Cells
  • 09:29Representative Microscopy Results
  • 10:44Conclusion

Summary

Tadução automática

Tadução automática

Here we demonstrate the protocols for performing single-molecule fluorescence microscopy on living bacterial cells to enable functional molecular complexes to be detected, tracked and quantified.

Tags

Advanced Fluorescence MicroscopySingle Molecular ComplexesIn Vivo VisualizationLiving CellsCellular MechanismsBiological SystemsSingle-molecule ExperimentationBulk Ensemble-average MeasurementsStochastic EventsHeterogeneous NatureOptical MicroscopyAnalytical Image Analysis ToolsMonitoring ProteinsLiving Bacterial CellMolecular ComplexesFunctioning Biological MachinesPhysiologically Relevant TechniquesMinimally-perturbativeNon-invasiveFluorescently-tagged Protein

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