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単一分子複合体の可視化インビボでの高度な蛍光顕微鏡を用いて
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Biologia
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JoVE Journal Biologia
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
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単一分子複合体の可視化インビボでの高度な蛍光顕微鏡を用いて

DOI:
10.3791/1508-v

•

11:26 min

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September 08, 2009

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, , ,
1Bioquímica,University of Oxford, 2Física,University of Oxford

Capítulos

  • 00:04Título
  • 01:02Introduction
  • 01:41Preparation of Bacteria
  • 03:49Preparing Cells for Microscopy
  • 07:16Visualizing and Analyzing the Cells
  • 09:29Representative Microscopy Results
  • 10:44Conclusion

Summary

Tadução automática

Tadução automática

ここでは、機能性分子複合体を追跡し、定量、検出できるようにするために細菌細胞を生きて単一分子蛍光顕微鏡を実行するためのプロトコルを示す。

Tags

Advanced Fluorescence MicroscopySingle Molecular ComplexesIn Vivo VisualizationLiving CellsCellular MechanismsBiological SystemsSingle-molecule ExperimentationBulk Ensemble-average MeasurementsStochastic EventsHeterogeneous NatureOptical MicroscopyAnalytical Image Analysis ToolsMonitoring ProteinsLiving Bacterial CellMolecular ComplexesFunctioning Biological MachinesPhysiologically Relevant TechniquesMinimally-perturbativeNon-invasiveFluorescently-tagged Protein

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