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Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
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Biologia
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JoVE Journal Biologia
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping

DOI:
10.3791/1512-v

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10:42 min

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September 04, 2009

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, , ,
1Institute of Biomedical Sciences,Federal University of Rio De Janeiro-UFRJ

Capítulos

  • 00:00Título
  • 00:03Introduction
  • 00:57Treating the Cells with Colecemid
  • 01:46Fixing the Cells
  • 07:00Washing the Cells
  • 08:11Preparing the Slides
  • 09:14Analyzing the Slides

Summary

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Karyotyping is a simple and useful technique widely used for detecting genetic alterations. Here we describe a step by step protocol for chromosome spread preparation of human embryonic stem cells for monitoring the chromosomal status of these cells maintained in culture.

Tags

Chromosomal Spread PreparationHuman Embryonic Stem CellsKaryotypingStable Diploid KaryotypeChromosomal AnomaliesCulture ConditionsEnzymatic DissociationChemical DissociationManual DissectionFeeder CellsGenetic IntegrityCellular PhysiologyEmbryogenesis StudiesDrug TestingTherapeutic PurposesChromosomal ChangesCarcinogenesisChromosome SpreadsG-bandingFISHSKY TechniquesCGH TechniquesTranslocationsAneuploidies

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