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SC1（Pluripotin）使用，以支持在没有LIF的自我更新MESC
JoVE Journal
Biologia
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JoVE Journal Biologia
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
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SC1（Pluripotin）使用，以支持在没有LIF的自我更新MESC

DOI:
10.3791/1550-v

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05:58 min

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November 18, 2009

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, , , , , , ,
1Research and Development,Stemgent, 2Product Marketing,Stemgent

Capítulos

  • 00:00Título
  • 00:08Introduction
  • 00:51Maintenance of Self-Renewal of Mouse Embryonic Stem Cells
  • 03:11Immunocytochemical Examination of Pluripotency Markers
  • 04:54Representative Images of Mouse Stem Cells Grown in the Presence of SC1
  • 05:28Conclusion

Summary

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Tadução automática

通过双抑制的Ras GAP和ERK1 SC1功能。我们SC1的功能，支持在没有LIF的小鼠ES细胞的自我更新和测试表明，SC1的是能够保持自我更新的小鼠胚胎干细胞培养。

Tags

SC1PluripotinMESC Self-renewalLIFSC1 Mechanism Of ActionRas-GAPERK1 InhibitionEconomical Alternative To LIFMolecular Mechanism Of Self-renewalCulturing Mouse ES Cells With SC1Morphology Of Mouse ES CellsPluripotency MarkersOct4Sox2NanogSSEA1Toxicity On Mouse ES Cells

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