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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
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Biologia
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JoVE Journal Biologia
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

DOI:
10.3791/1588-v

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12:11 min

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October 23, 2009

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, , , , , ,
1Department of Sciences and Chemical Technologies,University of Rome, Tor Vergata

Capítulos

  • 00:00Introduction
  • 00:30Competition Step
  • 01:46Measurement Step
  • 02:55Coating Step
  • 07:04Labeling Step
  • 11:59Conclusion

Summary

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A protocol to detect trichothecenes (mycotoxins of concern for human health) using a newly developed screening method based on a competitive immunochemical method and a final electrochemical detection is demonstrated.

Tags

ELIMEEnzyme Linked Immuno Magnetic ElectrochemicalMycotoxin DetectionImmunoassaysHPLCGCElectrochemical Competitive AssayMagnetic BeadsImmunochemical ChainScreen Printed ElectrodesHT-2 ToxinT-2 ToxinTrichothecenes FamilyHuman HealthAntibody CloneCross ReactivityCoating StepImmobilize HT2-KLH Conjugate ToxinBlocking StepMonoclonal AntibodyCompetition StepSample SolutionSecondary Antibody Labeled With Alkaline Phosphatase (AP)

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