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マウス胚性幹細胞から造血幹細胞の導出
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Biologia
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JoVE Journal Biologia
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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マウス胚性幹細胞から造血幹細胞の導出

DOI:
10.3791/162-v

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22:06 min

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February 25, 2007

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,
1Childrens Hospital, Harvard Stem Cell Institute,Harvard Medical School

Capítulos

  • 00:06Assembling handing drops differentiation culture
  • 00:08Collect undifferentiated ES cells
  • 02:24Prepare materials
  • 02:57Assemble hanging drop culture
  • 04:34Collecting differentiated ES cells form hanging drops
  • 06:05Starting EB culture
  • 06:53EB dissociation and infection
  • 11:25Preparation of ES cell-derived progenitors for FACS
  • 15:18Transplantation
  • 16:19Discussion

Summary

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Tadução automática

このプロトコルの詳細マウス胚性幹細胞（ESC）と致死量の放射線照射、レシピエントマウスへの彼らのその後の注射から移植造血幹細胞の派生。簡単に言うと、ESCは、レトロウイルスHoxB4とOP9ストローマ細胞と造血サイトカインとの共培養に感染している胚様体、として区別されます。

Tags

Stem CellsHematopoietic Stem CellsMurine Embryonic Stem CellsSelf-renewalDifferentiated ProgenyPluripotentDifferentiative AbilityClinically Useful Cell TypesVideo ProtocolHematopoietic MalignanciesHematopoietic DisordersHSC TherapyAlternative Source Of HSCBaselineHuman ESC IsolationEmbryoid Bodies (EBs)Serum Pre-screenedHematopoietic DifferentiationRetroviral HoxB4OP9 StromaBone Marrow Stromal Cell LineCytokines

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