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人类DNA修复蛋白的遗传研究利用酵母作为模型系统
JoVE Journal
Biologia
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JoVE Journal Biologia
Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System
DOI:

14:09 min

March 18, 2010

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Capítulos

  • 00:00Título
  • 01:15Introduction
  • 03:01Plasmid DNA Constructions
  • 04:04Streak Analysis of the Slow Growth Phenotype Restoration in WRN Transformed sgs1 top3 Strain
  • 06:00Genetic Analysis of Hydroxyurea (HU) or Methylmethane-sulfonate (MMS) Sensitivity in WRN Transformed Yeast Strains
  • 07:24Determining Cell Cycle Distribution of the WRN Transformated sgs1 top3 Strain
  • 09:21Preparing Cell Lysates for Western Blot Analysis
  • 11:09Effects of WRN Expression in sgs1 and top3 Yeast Cells
  • 13:23Conclusion

Summary

Tadução automática

可在酵母的遗传研究调查的人类基因在细胞DNA的代谢的分子和细胞的功能。方法描述为人类的遗传特征<em> WRN</em>早衰障碍沃纳综合征在使用酵母作为一个听话的模型系统的功能保守的途径有缺陷的基因产物。

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