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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
 
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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Article DOI: 10.3791/1662-v • 12:56 min • January 7th, 2010
January 7th, 2010 •
1, 1, 1
1Gene Expression Division, Bio-Rad Laboratories, Inc.

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This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.

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Gene Pulser MXcell Electroporation System Transfect Primary Cells High Efficiency Plasmid DNA SiRNA Electroporation Buffer Bio-Rad Nucleic Acids Mammalian Cells Difficult-to-transfect Cells Stem Cells Electroporation Conditions Optimization 96-well Electroporation Plates Electroporation Cuvettes Electroporation Efficiency Success Or Failure Of Electroporation Experiments
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