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Capítulos
- 00:00Título
- 00:58Introduction
- 01:23Preparation of the Plant Material
- 02:43Harvesting and Protoplasting of the Plant Material
- 06:10Fluorescence Activated Cell Sorting of Protoplasts
- 11:26FACS Followed by RNA Extraction of Plant Protoplasts Representative Results
- 12:59Conclusion
En metod för att isolera specifika celltyper från växtmaterial visas. Denna teknik använder transgena markör som uttrycker fluorescerande proteiner i synnerhet celltyper, cellulär dissociation och fluorescens aktiverad cell sortering. Dessutom är en tillväxt inställning etablerade här som underlättar behandlingen av<em> Arabidopsis thaliana</em> Plantor innan cellen sortering.
Tags
Fluorescence Activated Cell SortingPlant ProtoplastsGene Expression AnalysisDevelopmental RegulationEnvironmental StimuliIn Situ HybridizationReporter Gene VisualizationQuantitative RT-PCRHigh-throughput Transcriptome-wide AnalysisRNA IsolationCell TypesCDNA Synthesis/amplificationMicroarray AnalysisFluorescent Protein MarkerArabidopsis Thaliana RootPSCR::GFPPWOX5::GFPHydroponicallyAgar PlatesEnzymatic Digestion Of Cell Wall
