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Mesure cérébrale oxygénation sanguine à base d'oxygène-dépendant trempe de phosphorescence
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1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology,Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics,University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology,Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology,University of California
Capítulos
- 00:05Título
- 00:11Introduction
- 00:56Wide Field of View Imaging of Partial Pressure of Oxygen in Cortical Vasculature of a Rat
- 04:55High Resolution Two-photon pO2 Imaging in Cortical Micro Vasculature of a Mouse
- 08:22Conclusion
Nous présentons une procédure expérimentale pour mesurer la pression partielle d'oxygène (pO2) dans le système vasculaire cérébrale à base d'oxygène-dépendant trempe de phosphorescence. La préparation des animaux et des procédures d'imagerie ont été décrites pour les deux grand champ de vue CCD à base d'imagerie de pO2 chez le rat et l'imagerie 2-photons d'excitation basée sur pO2 chez la souris.
Tags
Cerebral Blood Oxygenation MeasurementOxygen-dependent Quenching Of PhosphorescenceSpatiotemporal CharacteristicsNeuro-metabolic-vascular RelationshipPO2 Measurement ModalitiesLarger Fields Of ViewHigher Spatial ResolutionHigher Temporal ResolutionNormal Brain FunctioningNeurovascular DiseasesStrokeAlzheimer’s DiseaseHead InjuryOptical Imaging ModalitiesHemoglobin AbsorptionVisible And Near Infrared RangeMultispectral MeasurementBlood Oxygenation EstimationPartial Pressure Of Oxygen (pO2)Oxygen-sensitive Dyes
