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Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations
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Biologia
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JoVE Journal Biologia
Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

DOI:
10.3791/1696-v

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08:35 min

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February 12, 2010

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, , ,
1Department of Biology and Biochemistry,University of Bath, 2Randall Division of Cell and Molecular Biophysics,King’s College London

Capítulos

  • 00:00Título
  • 01:06Introduction
  • 01:43Preparation
  • 02:50Procedure for Mounting Drosophila Embryos
  • 07:03Representative Results
  • 07:55Conclusion

Summary

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Drosophila hemocytes disperse over the entirety of the developing embryo. This protocol demonstrates how to mount and image these migrations using embryos with fluorescently labelled hemocytes.

Tags

Live ImagingDrosophila MelanogasterEmbryonic Hemocyte MigrationsIn Vitro MethodsPhysiologically Relevant EnvironmentMounting ProtocolFluorescently Labeled HemocytesGal4-uas SystemGenetically Encoded MarkersDevelopmental DispersalEmbryo CollectionOuter Chorion RemovalHalocarbon OilHydrophobic MembraneGlass CoverslipGross Migratory ParametersSpeedDirectionalityHigher Resolution ImagingF-actinMicrotubulesCytoskeletal Components

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