É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:30Introduction
- 01:03Preparing for Microinjection
- 03:14Microinjection and Oocyte Culture
- 04:35Fixation, Dehydration, and Storage of Oocytes
- 05:35Imaging RNA Localization by Confocal Microscopy
- 05:59RNA Localization in Xenopus Oocytes: Representative Results
- 06:28Conclusion
Tags
RNA LocalizationXenopus OocytesCell PolarityVg1 MRNAGene ExpressionGerm Layer SpecificationRNA Sequence ElementsVg1 Localization Element (VLE)Trans-factor Directed TransportImaging TechniqueFluorescently Labeled VLE RNAMicroinjectionConfocal MicroscopyMRNA Localization PatternsCis-acting ElementsTrans-acting FactorsCo-localizationMotor ProteinsCytoskeleton
