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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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Biologia
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JoVE Journal Biologia
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

DOI:
10.3791/1712-v

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22:00 min

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November 21, 2010

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1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine,Trinity College Dublin

Capítulos

  • 00:00Introduction
  • 02:48Materials
  • 03:48Preparing the Crystallization Plate
  • 05:12Preparing the Lipid and Protein
  • 13:53Loading the Dispensing Syringe
  • 17:57Dispensing
  • 21:10Crystals

Summary

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Tadução automática

Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.

Tags

Crystallizing Membrane ProteinsLipidic MesophasesLipidic Cubic PhaseIn Meso MethodX-ray Crystallographic StructuresProkaryotic ProteinsEukaryotic ProteinsMonomeric ProteinsHomo-multimeric ProteinsHetero-multimeric ProteinsChromophore-containing ProteinsChromophore-free ProteinsAlpha-helical ProteinsBeta-barrel ProteinsBeta2-adrenergic ReceptorAdenosine A2a ReceptorReconstituting Membrane ProteinMonoolein-based MesophaseManual Crystallization SetupCrystal Harvesting

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