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A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
 

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

Article DOI: 10.3791/1720-v • 09:58 min • April 13th, 2010
April 13th, 2010 •
1, 1, 1, 2, 1,3, 1,3
1Department of Medicine, Baylor College of Medicine (BCM), 2Millenium Premier Group, 3Department of Immunology, Baylor College of Medicine (BCM)

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Resumo
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Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.

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Reversible Non-invasive Method Airway Resistance Measurements Bronchoalveolar Lavage Fluid Sampling Mice Airway Hyperreactivity Experimental Asthma Models Repeated Procedures AHR Measurements BAL Fluid Sampling Protocols Intranasal Challenge Allergen Sham Treatment Anesthetized Orally Intubated Mechanically Ventilated Dose Response Curves Respiratory System Resistance (RRS) Acetylcholine (Ach) Chloride Allergen-challenged Animals Differential Enumeration Of Airway Cells Repeated Measurements Of AHR And BAL Fluid Collection Maximal Airway Hyperresponsiveness Airway Eosinophilia Allergen Challenge Longitudinal Experimentation
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