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在斑马鱼胚胎的单个神经元和神经嵴细胞的细胞运动和肌动蛋白细胞骨架的实时成像
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Biologia
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JoVE Journal Biologia
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
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在斑马鱼胚胎的单个神经元和神经嵴细胞的细胞运动和肌动蛋白细胞骨架的实时成像

DOI:
10.3791/1726-v

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10:52 min

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February 03, 2010

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, , ,
1Genetics Training Program,University of Wisconsin-Madison, 2Department of Anatomy,University of Wisconsin-Madison, 3Department of Zoology,University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program,University of Wisconsin-Madison

Capítulos

  • 00:00Título
  • 00:47Introduction
  • 01:18Assembling Injection Slides and Imaging Slides
  • 02:36Injecting DNA Constructs
  • 04:43Mounting Embryos for Imaging
  • 06:524D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
  • 08:49Behavior of Individual Labeled Neurons and Neural Crest Cells
  • 10:23Conclusion

Summary

Tadução automática

Tadução automática

本协议描述了生活的斑马鱼胚胎的单个神经元或神经嵴细胞成像。使用此方法来研究细胞行为和肌动蛋白定位使用荧光共焦时间推移显微镜。

Tags

Live ImagingCell MotilityActin CytoskeletonIndividual NeuronsNeural Crest CellsZebrafish EmbryosDevelopmentIn VivoOptical ClarityHigh Resolution ImagingCell BehaviorsMigrationAxon OutgrowthGuidanceMechanismsCell LabelingPlasmid DNA InjectionTransient Mosaic Expression PatternProtrusive ActivityMolecular DynamicsTransgenic LinesFine ProtrusionsChanges In Molecular Distribution

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