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Murine Model of CD40-activation of B cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Murine Model of CD40-activation of B cells

Murine Model of CD40-activation of B cells

DOI:
10.3791/1734-v

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12:24 min

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March 05, 2010

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, , , ,
1Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine,University Hospital of Cologne

Capítulos

  • 00:00Título
  • 00:14Introduction
  • 02:10Preparation of Murine Splenocytes for CD40 – Stimulation
  • 04:19Preparation of Feeder Cells
  • 07:59CD40 – Stimulation
  • 08:50Subcultivation
  • 11:25Analysis of Murine CD40 – Activated B Cells
  • 11:47Conclusion

Summary

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In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.

Tags

CD40-activationB CellsAntigen PresentationInterleukin-4CD40 LigandExpansionPeripheral BloodCD40-B CellsLymph Node Homing MoleculesT CellsAntigensMemory T CellsImmuno-stimulatory Antigen-presenting Cells (APC)Cell-based ImmunotherapyMurine CD40-activated B CellsSplenocytesPurified B CellsC57BL/6 MiceHaplotype H-2bDensity Gradient CentrifugationHeLa CellsRecombinant Murine CD40 Ligand (tmuCD40L HeLa)

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