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果蝇聚乙烯南瓜抗体标记染色体的制备
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Biologia
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JoVE Journal Biologia
Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
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果蝇聚乙烯南瓜抗体标记染色体的制备

DOI:
10.3791/1748-v

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06:37 min

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February 09, 2010

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, , , ,
1Department of Biochemistry, Biophysics, and Molecular Biology,Iowa State University

Capítulos

  • 00:00Título
  • 00:28Introduction
  • 01:05Culture of Third Instar Drosophila Larvae
  • 02:04Squash Preparation of Polytene Chromosomes
  • 05:06Representative Results
  • 06:13Conclusion

Summary

Tadução automática

Tadução automática

该视频协议说明在约翰森实验室中使用的壁球技术准备<em>果蝇</em>聚乙烯抗体标记染色体。

Tags

DrosophilaPolytene ChromosomesAntibody LabelingChromatin StructureGene RegulationCytological AdvantagesGiant Salivary GlandThird Instar LarvaeReplicative CycleChromatin MorphologyEpigenetic ModificationsTranscription ProcessLabeling Technique

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