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分离人脐静脉内皮细胞（HUVEC）
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Biologia
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JoVE Journal Biologia
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
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分离人脐静脉内皮细胞（HUVEC）

DOI:
10.3791/183-v

•

09:19 min

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April 28, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI)

Capítulos

  • 00:08Introduction
  • 00:52Preparation of umbilical cord
  • 02:00Digestion of umbilical vein and isolation of HUVEC

Summary

Tadução automática

Tadução automática

这个视频协议说明了人脐静脉内皮细胞（HUVEC）的人脐血的分离和培养。一旦分离出这些细胞可以在体外血管生成像优化的纤维蛋白凝胶珠还展示了由休斯实验室的检测分析。

Tags

IsolationHuman Umbilical Vein Endothelial CellsHUVECAngiogenesisMulti-step ProcessVascular DegradationProliferationMigrationSproutingExtracellular MatrixAlignmentCord FormationLumen FormationAnastomosisNew Basement MembraneIn Vitro AssaysMorphological ResemblanceOptimized In Vitro Angiogenesis AssayHuman Umbilical Vein EC And FibroblastsEarly Stages Of AngiogenesisIntercellular LumensPolarized ECPhase-contrast MicroscopyTime-lapse MicroscopyDownstream Applications

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