Harvesting Sperm and Artificial Insemination of Mice

Amanda R. Duselis; Paul B. Vrana

doi:10.3791/184

Journal

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Biologia

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Harvesting Sperm and Artificial Insemination of Mice

JoVE Journal
Biologia

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JoVE Journal Biologia

Harvesting Sperm and Artificial Insemination of Mice

DOI:

10.3791/184-v

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10:00 min

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April 28, 2007

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Amanda R. Duselis, Paul B. Vrana

¹Dept. of Biological Chemistry,University of California, Irvine (UCI)

Capítulos

00:06Introduction
01:09Tools and Reagents
02:17Dissection procedure
04:43Removal of epididymis
07:58Visualization of sperm
08:20Insemination of females
09:27Conclusion

Summary

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This protocol demonstrates methods for extracting sperm from the testes of males and then inseminating female mice. This procedure is useful when precise time is needed in developmental studies as well as transgenic work.

Harvesting Sperm and Artificial Insemination of Mice

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Capítulos

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