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Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
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Biologia
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JoVE Journal Biologia
Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41

Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41

DOI:
10.3791/1844-v

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11:07 min

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March 25, 2010

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,
1Department of Life Sciences and National Institute for Biotechnology in the Negev,Ben-Gurion University

Capítulos

  • 00:00Título
  • 00:42Introduction
  • 01:10MamA∆41 Protein Expression
  • 02:21MamA∆ 41 Proteing Extraction
  • 05:26Fast Performance Liquid Chromatography (FPLC)
  • 07:57MamA∆41 Protein Purity Evaluation
  • 08:50Representative Results of MamA∆41 Protein Purification
  • 10:40Conclusion

Summary

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Tadução automática

MamA is a unique Magnetosome associated protein which was shown to be involved in magnetosome activation. Here we present the purification protocol of MamA deletion mutant (MamAΔ41) from M. magneticum AMB-1.

Tags

PurificationM. MagneticumStrain AMB-1Magnetosome Associated ProteinMamAΔ41Magnetotactic BacteriaGeomagnetic FieldsMagnetosomeLipid VesiclesBiomineralizeMagnetiteGreigiteFunctional VesiclesMagnetosome-associated ProteinPurification Of MamAFunctional DetailsStructural DetailsBioinformatics AnalysisTetra-tricopeptide Repeat (TPR)Protein-protein InteractionsMulti-protein ComplexesEukaryotic Cell Organelle ProcessesBacterial Pathways

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