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分离和富集大鼠间质干细胞（MSCS）及衍生的干细胞分离单细胞集落

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

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分离和富集大鼠间质干细胞（MSCS）及衍生的干细胞分离单细胞集落

DOI:

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08:18 min

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March 22, 2010

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Linxia Zhang, Christina Chan

¹Department of Chemical Engineering and Materials Science,City of Hope Cancer Center

Capítulos

00:00Título
00:47Introduction
01:18Isolation of Rat MSCs
02:40Enrichment of Rat MSCs
05:17Separation of Single-colony Derived MSCs
06:36Results of Isolation and Enrichment of Rat MSCs
07:41Conclusion

Summary

Tadução automática

大鼠MSCs从股骨和胫骨分离，然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养，形成单菌落，然后这些菌落克隆气瓶分开。

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