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Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
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Biologia
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JoVE Journal Biologia
Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast

DOI:
10.3791/1865-v

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09:34 min

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March 30, 2010

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,
1Department of Plant Sciences,Tel Aviv University

Capítulos

  • 00:00Título
  • 00:37Introduction
  • 01:10Designing a Yeast Expression System for Type III Effectors
  • 02:13Yeast Transformation
  • 04:21Preparing Yeast Protein Extracts to Verify Effector Experession
  • 06:46Spotting Assay to Detect Effector-induced Growth Inhibition Phenotypes
  • 08:08Spotting Assay Results
  • 09:02Conclusion

Summary

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In this video, we describe a procedure for the expression of bacterial type III effectors in yeast and the identification of effector-induced growth inhibition phenotypes. Such phenotypes can be subsequently exploited to elucidate effector functions and targets.

Tags

Growth Inhibition PhenotypesBacterial Type III EffectorsGram-negative Pathogenic BacteriaType III Secretion SystemEffector ProteinsHost CellsImmune ResponsesPathogen GrowthEffector RepertoireVirulenceInfection StagesGenetic ManipulationBudding Yeast Saccharomyces CerevisiaeFunctional Characterization Of Effector ProteinsCellular ProcessesEukaryotes

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