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DNA-based Fish Species Identification Protocol
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

DOI:
10.3791/1871-v

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09:15 min

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April 28, 2010

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, , , , , ,
1Agilent Technologies

Capítulos

  • 00:00Título
  • 00:43Introduction
  • 01:34Preparation of Fish Samples for DNA Extraction
  • 02:22Genomic DNA Extraction
  • 04:26PCR and Restriction Enzyme Digest
  • 05:58Identification of Test Sample Species using the Agilent Bioanalyzer and RFLP Decoder Software
  • 08:14Representative Analysis of Four Distinct Fish Species
  • 08:44Conclusion
  • 09:14Credits

Summary

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This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.

Tags

DNA-basedFish Species IdentificationScreening MethodPCR AmplificationGenomic DNARestriction Fragment Length Polymorphism (RFLP) AnalysisAgilent 2100 BioanalyzerRFLP Pattern Matching SoftwareSpin Column-based ProtocolNucleic AcidsBinding BufferMicro-spin CupSilica-based Fiber MatrixPCR AmplificationPrimersRestriction EnzymesDigestion ReactionsDatabase Of Experimental RFLP Patterns

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