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Capítulos
- 00:00Título
- 00:47Introduction
- 01:22Producing the GAL4 Enhancer-trap Line
- 02:37Plant Growth and Leaf Protoplasts Isolation
- 07:18Protoplast Sorting by Fluorescence-Activated Cell Sorting (FACS)
- 08:47Leaf Protoplasts Isolation and Sorting by FACS Representative Results
- 09:38Conclusion
The molecular basis of spatial-specific phytochrome responses is being investigated using transgenic plants that exhibit tissue- and organ-specific phytochrome deficiencies. The isolation of specific cells exhibiting induced phytochrome chromophore depletion by Fluorescence-Activated Cell Sorting followed by microarray analyses is being utilized to identify genes involved in spatial-specific phytochrome responses.
