É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Introduction
- 00:38First step: starving the fly
- 02:06Fixing the fly
- 04:00Preparing the wick
- 06:02Where to make contact on labellum
- 10:37How to make contact
- 14:42Positive and negative controls
- 24:16Discussion
Proboscis extension response or PER is a taste behavior assay that has been used in flies as well as in honeybees. When the proboscis makes contact with an attractive substance, the fly extends its proboscis to consume the substance. Solutions of various sugars are very attractive to the fly.
Tags
Proboscis Extension Response (PER) AssayTaste BehaviorFliesHoneybeesLabellumSensillaTaste NeuronsAttractive SubstanceProboscis ExtensionFood PreferencesSugarsSucroseGlucoseFructoseAversive SubstancesSweet SolutionResponsive StateStarvation TimeControlsNon-responsiveHyper-responsiveImpact LevelContact PositionReproducibility
