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Biologia
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Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)
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Biologia
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JoVE Journal Biologia
Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

DOI:
10.3791/1940-v

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08:06 min

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July 03, 2010

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, , ,
1Centro de Investigação em Ciências da Saúde, Faculdade de Ciências da Saúde,Universidade da Beira Interior

Capítulos

  • 00:00Título
  • 00:06Solutions
  • 00:48Preparation of Umbilical Cords
  • 02:40Digestion of Umbical Arteries and Isolation of Smooth Muscle Cells
  • 04:51Culture
  • 08:00Credits

Summary

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The umbilical cords are used to isolate smooth muscle cells by different ways. In this work we used the enzymatic treatment to isolated smooth muscle cells.

Tags

IsolationHuman Umbilical Arterial Smooth Muscle CellsHUASMCUmbilical CordBiological SampleEndothelial CellsSmooth Muscle CellsBlood VesselsEnzymatic TreatmentExperimental ProcedureCell IsolationT-flashDMEM-F12Fetal Bovine SerumCulturePassagesMorphological CharacteristicsPhenotypic CharacteristicsConstrictor DrugsL-type Calcium ChannelsVascular Function

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