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人多能干细胞的免疫组织化学分析，使用自制Cytospin器械

JoVE Journal
Biologia
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JoVE Journal Biologia

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

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人多能干细胞的免疫组织化学分析，使用自制Cytospin器械

DOI:

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09:16 min

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April 09, 2010

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Enrique Y. Pascual, Marion J. Riggs, Raj R. Rao

¹Department of Chemical and Life Sciences Engineering,Virginia Commonwealth University

Capítulos

00:00Titlle
00:12Introduction
08:26Representative Results
08:42Conclusion
09:02Credits

Summary

Tadução automática

暂停人类多能干细胞的细胞表面标记的免疫细胞化学染色（hPSCs）（SSEA-3/SSEA-4）是实现的基础上使用自制的cytospin设备创建一个观察和定量细胞单层。

Tags

Immunocytochemical Analysis Human Pluripotent Stem Cells HPSCs Human Embryonic Stem Cells HESCs Human Induced Pluripotent Stem Cells HiPSCs Self-made Cytospin Apparatus Cell Sources Self-renewal Capabilities Differentiation QPCR In Vitro Differentiation In Vivo Differentiation Intracellular Biomarkers Cell-surface Biomarkers Colony Growth Marker Evaluation Monolayer Cell Samples

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